Strand-specific real-time RT-PCR for distinguishing influenza vRNA, cRNA, and mRNA
نویسندگان
چکیده
منابع مشابه
Development and applications of a real-time quantitative RT-PCR method (QRT-PCR) for BRCA1 mRNA.
OBJECTIVES To develop a real-time quantitative RT-PCR method for BRCA1 mRNA and then use it for the study of BRCA1 gene expression in human MCF-7 breast cancer cells after their exposure to antineoplastic agents and gamma irradiation. DESIGN AND METHODS The developed QRT-PCR method is based on the real-time monitoring of a fluorescein-labeled TaqMan probe, specific for BRCA1 mRNA, during PCR ...
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ژورنال
عنوان ژورنال: Journal of Virological Methods
سال: 2011
ISSN: 0166-0934
DOI: 10.1016/j.jviromet.2010.12.014